[Histonet] Kidney Bx question

[kemlo]

I'd get agitated if I was processing unfixed tissue (15 min?) so quickly
<g>.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 20 March 2008 12:18
To: Marshall Terry Dr,Consultant Histopathologist; histoinfo <@t> comcast.net;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Kidney Bx question

For hand processing at room temperature that schedule is too short, unless
the samples are agitated.
  René J.

"Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk> wrote:
  Do you have a train to catch?

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histoinfo <@t> comcast.net
Sent: 19 March 2008 17:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Kidney Bx question

We have been having some trouble with the inner surface of the tubules
looking torn look to the tissue. We hand process all our kidney bx's
with the times as follows.

1-formalin 15 minutes
2-50% ethanol 15 minutes
3-70% ethanol 15 minutes
4-80% ethanol 15 minutes
5-95% ethanol 15 minutes
6-100% ethanol 15 minutes
7-100% ethanol 15 minutes
9-xylene 15 minutes
10-xylene 15 minutes
Parraffin x2, 20 minutes each. 
They are then cut at 1 micron for PAS & JONES and 2 microns for H&E &
TRICHROME. 

We are trying to determine if this tearing is an artifact from
processing, collection or some other factor we haven't thought of. Has
anyone else come across this or a similar problem. All thoughts and
suggestions welcome.

Jennifer Saunders HT (ASCP)
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