[Histonet] Barrier/Pap Pen

[Gayle Callis]

Try Immedge pens from Vector.  Cheaper, two in a set.   When you use this 
pen,  and maybe even your pens, shake the begollys out of the pen to 
redistribute the ingredients per advice from Vector.  We vortex ours before 
using - on highest speed, and make sure the goo is coming out by touching 
tip to paper.  Too much goo is a disaster.

 If you are making the barrier after the slide surface is wet, make sure it 
is dry where you want the barrier.  We make straight line barriers rather 
than circles to blot from a corner - easier.   The Tween may be causing some 
of the problem so making a mark on slide that has not been immersed in 
buffer with Tween may help.  Beware, the section could dry though.

Gayle Callis
HTL/HT/MT(ASCP)
Bozeman MT

----- Original Message ----- 
From: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, March 18, 2008 5:27 PM
Subject: [Histonet] Barrier/Pap Pen


Dear Histoneters,



I am having problems with my pap pen.  I have been using the pap pen from 
VWR
called the "Liquid Blocker super pap pen." It's terrible; every time I wash 
in
Tween 20/PBS it loses it's ability to make a hydrophobic barrier, which mean 
I'm
constantly patching the barrier before adding my primary, secondary, and
developing steps.  I found one made by Ambion cat# 15500G which works great 
but
they only sell these in a 150$ LCM kit.  Does anyone know who Ambion gets 
theirs
from?  Or does anyone have recommendations of a pap pen they like the best?
Thanks for your help!



Sam Perry



Research Technician

Dana Farber Cancer Institute

Boston MA 02115


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