[Histonet] Barrier/Pap Pen
gayle.callis <@t> bresnan.net
Tue Mar 18 19:11:07 CDT 2008
Try Immedge pens from Vector. Cheaper, two in a set. When you use this
pen, and maybe even your pens, shake the begollys out of the pen to
redistribute the ingredients per advice from Vector. We vortex ours before
using - on highest speed, and make sure the goo is coming out by touching
tip to paper. Too much goo is a disaster.
If you are making the barrier after the slide surface is wet, make sure it
is dry where you want the barrier. We make straight line barriers rather
than circles to blot from a corner - easier. The Tween may be causing some
of the problem so making a mark on slide that has not been immersed in
buffer with Tween may help. Beware, the section could dry though.
----- Original Message -----
From: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, March 18, 2008 5:27 PM
Subject: [Histonet] Barrier/Pap Pen
I am having problems with my pap pen. I have been using the pap pen from
called the "Liquid Blocker super pap pen." It's terrible; every time I wash
Tween 20/PBS it loses it's ability to make a hydrophobic barrier, which mean
constantly patching the barrier before adding my primary, secondary, and
developing steps. I found one made by Ambion cat# 15500G which works great
they only sell these in a 150$ LCM kit. Does anyone know who Ambion gets
from? Or does anyone have recommendations of a pap pen they like the best?
Thanks for your help!
Dana Farber Cancer Institute
Boston MA 02115
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