[Histonet] Barrier/Pap Pen

[Perry, Samuel]

Dear Histoneters,

 

I am having problems with my pap pen.  I have been using the pap pen from VWR
called the "Liquid Blocker super pap pen." It's terrible; every time I wash in
Tween 20/PBS it loses it's ability to make a hydrophobic barrier, which mean I'm
constantly patching the barrier before adding my primary, secondary, and
developing steps.  I found one made by Ambion cat# 15500G which works great but
they only sell these in a 150$ LCM kit.  Does anyone know who Ambion gets theirs
from?  Or does anyone have recommendations of a pap pen they like the best?
Thanks for your help!  

 

Sam Perry

 

Research Technician

Dana Farber Cancer Institute

Boston MA 02115


The information transmitted in this electronic communication is intended only
for the person or entity to whom it is addressed and may contain confidential
and/or privileged material. Any review, retransmission, dissemination or other
use of or taking of any action in reliance upon this information by persons or
entities other than the intended recipient is prohibited. If you received this
information in error, please contact the Compliance HelpLine at 800-856-1983 and
properly dispose of this information.