[Histonet] Barrier/Pap Pen

Perry, Samuel Samuel_Perry <@t> DFCI.HARVARD.EDU
Tue Mar 18 18:27:11 CDT 2008

Dear Histoneters,


I am having problems with my pap pen.  I have been using the pap pen from VWR
called the "Liquid Blocker super pap pen." It's terrible; every time I wash in
Tween 20/PBS it loses it's ability to make a hydrophobic barrier, which mean I'm
constantly patching the barrier before adding my primary, secondary, and
developing steps.  I found one made by Ambion cat# 15500G which works great but
they only sell these in a 150$ LCM kit.  Does anyone know who Ambion gets theirs
from?  Or does anyone have recommendations of a pap pen they like the best?
Thanks for your help!  


Sam Perry


Research Technician

Dana Farber Cancer Institute

Boston MA 02115

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