[Histonet] Endogenous Peroxide Treatment - Immunohistochemistry
imilos <@t> cellmarque.com
Sat Mar 15 13:29:08 CDT 2008
If you are running your IHC stains with an HRP detection kit with a
biotinylated secondary antibody, endogenous biotin within the tissue can
cause background staining to occur. Often times, you'll see endogenous
biotin in a tissue that is particularly bloody, such as liver, kidney,
You can use an avidin/biotin block before secondary antibody is applied
to account for this. You can find this block through many different
companies - mine (Cell Marque) offers a good version. The part number
is CMX222 and it can be ordered at 1-800-665-7284.
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marilyn
Sent: Saturday, March 15, 2008 11:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry
For routine tissues, I use 3% hydrogen peroxide for 10 mins. to remove
Presently, I am trying to stain liver tissue, which stores more of the
There seems to be background staining that masks blood cells and
I've tried using 5% hydrogen peroxide solution for 10 mins. and there is
Are there other solutions that can be used instead of hydrogen peroxide?
Any replies would be greatly appreciated.
Thank you in advance.
Food and Safety Division
Edmonton, Alberta, Canada.
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