[Histonet] Endogenous Peroxide Treatment - Immunohistochemistry

Marilyn Johnson marjoh3 <@t> telus.net
Sat Mar 15 13:26:36 CDT 2008

Hi Histonetters,
For routine tissues, I use 3% hydrogen peroxide for 10 mins. to remove RBC's.
Presently, I am trying to stain liver tissue, which stores more of the blood components.
There seems to be background staining that masks blood cells and vessels.
I've tried using 5% hydrogen peroxide solution for 10 mins. and there is no improvement.
Are there other solutions that can be used instead of hydrogen peroxide?
Any replies would be greatly appreciated.
Thank you in advance.

Marilyn Johnson
Alberta Agriculture
Food and Safety Division
Edmonton, Alberta, Canada.

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