[Histonet] bodians stain

Bryan Llewellyn llewllew <@t> shaw.ca
Thu Mar 6 14:04:35 CST 2008

Protargol is a protein-silver complex and is a very variable material.  We 
had the same problem many years ago.  The resolution was to get several 
samples of protargol from different sources and choose one that worked. 
Also, make sure you use copper shot about 2 mm diameter.

Bryan Llewellyn

----- Original Message ----- 
From: "Bell, Mandy" <Mandy.Bell <@t> chomp.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, March 06, 2008 11:52 AM
Subject: [Histonet] bodians stain

Does anyone have any tips on getting the Bodian method stain to work?  My 
coworker has been trying for the past few days, and the tissue is not 
turning dark tan to brown in the protargol solution.  We've tried 
microwaving and letting it sit in the warm solution for 20 min, and 
repeating this multiple times, and just letting it sit in the protargol 
overnight.  The only thing we've thought of is that the protargol solution 
may have been shaken up, which the procedure says not to do.  Any tips would 
be greatly appreciated.  (We're using FFPE brain tissue cut at 8 microns) 

Mandy M Bell
Histology Department

Community Hospital of the Monterey Peninsula


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