[Histonet] IHC staining of previously H&E-stained prostate cores

Sebree Linda A. LSebree <@t> uwhealth.org
Mon Jun 23 10:47:27 CDT 2008

We haven't specifically restained prostate bxs for IHC but we have IHC
stained over H&E staining on other tissues.  We remove the coverslip
tape with a 50/50 xylene/acetone mix, then run down through xylene and
graded alcohols to remove any remaining mounting media and hydrate to
water.  From here we use our usual protocol minus any deparaffinization
step.  This has always worked fine for us unless the original H&E slide
was not a "+" slide in which case we sometimes lose tissue.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
JMyers1 <@t> aol.com
Sent: Monday, June 23, 2008 10:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC staining of previously H&E-stained prostate

I'm trying to stain previously H&E-stained prostate core biopsies  for
and P504S, and I've been obtaining less than ideal results.  I  suspect
this might be caused by inadequate removal of mounting  media (once the 
tape-coverslip is removed with acetone) or over/under-  retrieval, but I
really use some advice from someone who has already  worked through a
like this.

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