[Histonet] IHC staining of previously H&E-stained prostate cores

[JMyers1 <@t> aol.com]

I'm trying to stain previously H&E-stained prostate core biopsies  for HMW-CK 
and P504S, and I've been obtaining less than ideal results.  I  suspect that 
this might be caused by inadequate removal of mounting  media (once the 
tape-coverslip is removed with acetone) or over/under-  retrieval, but I could 
really use some advice from someone who has already  worked through a situation 
like this.




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