[Histonet] IHC staining of previously H&E-stained prostate cores

godsgalnow <@t> aol.com godsgalnow <@t> aol.com
Mon Jun 23 10:47:46 CDT 2008


When we have to do this, we let the slides soak in xylene until the cover slip fails off.? We then, run a de-wax program for the slides to hydrate them and then treat them like any other IHC.? You may or may not need to counter stain them when they are finished.? Should work great.


-----Original Message-----
From: JMyers1 <@t> aol.com
To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, 23 Jun 2008 11:03 am
Subject: [Histonet] IHC staining of previously H&E-stained prostate cores

I'm trying to stain previously H&E-stained prostate core biopsies  for HMW-CK 
and P504S, and I've been obtaining less than ideal results.  I  suspect that 
this might be caused by inadequate removal of mounting  media (once the 
tape-coverslip is removed with acetone) or over/under-  retrieval, but I could 
really use some advice from someone who has already  worked through a situation 
like this.

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