[Histonet] Animal Tissue processing question

Rosa Fields rfields <@t> gidocs.net
Thu Jun 19 09:30:11 CDT 2008

I would have to agree with Victor, also previously from a high volume vet diagnostic lab, as a matter of practicality all of our species were also grouped onto one processor.  It is very challenging to practically deal with everything one may see in a vet diagnostic lab! 

Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE  68503
rfields <@t> gidocs.net

The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.  If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Thursday, June 19, 2008 9:01 AM
To: Rene J Buesa
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Animal Tissue processing question

Having worked previously in a high volume vet diagnostic lab I somewhat 
disagree with Rene. For optimal processing I agree, but not for 
practicality. On a daily basis we would have every species under the sun 
(almost) and they got grouped together on one processor. Some days 
certain tissues might be a little more difficult, but not impossible.


Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-7659 Fax
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.

Rene J Buesa wrote:
> You just mentioned 2 different species with peculiar characteristics. The difficulty with veterinary histology is that you will have to modify your procedures slightly to each, and you should never process tissues of different species in the same run.
>   From very "dry" mice tissues with less dehydration times, to pig tissues requiring more time in the antemedium because of the tisular fat (unless it is a boar) you will have to prepare modified protocol.
>   My advise, write down your protocols and the animals you used them with and evaluate the results in a manner that in some time you will have the protocols you need.
>   Sorry if this is not what you asked for, but, as I wrote you, there should be specific protocols.
>   René J.
> histoinfo <@t> comcast.net wrote:
>   Dear Histonetters,
> After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help.
> Jennifer
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list