[Histonet] (no subject)

Aine Behan abehan <@t> rcsi.ie
Tue Jun 17 11:42:31 CDT 2008

I was looking for advice on troubleshooting already cryoprotected tissue for
freezing artefact (FA). 


I have mice brains dissected out after cervical dislocation, postfixed in 4%
Paraformaldehyde/PBS overnight (4 degrees), cryoprotected in 30% sucrose for
~24 hours (4 degrees) and then placed in -80 freezer. This FA never was an
issue before and the animals are treatment free so anyone know why I would
be getting this now?





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