[Histonet] Fite stain

[Bryan Llewellyn]

Sometimes an old carbol fuchsin solution will leave tiny droplets on the 
slide.  This may be the source of the problem.  Make up fresh carbol 
fuchsin, or buy it, and try again.

If you are doing the Fite stain for TB organisms and not for Leprosy in 
particular, why not consider switching to a regular ZN.

Bryan
Llewellyn


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, 11 June 2008 10:57 PM
To: histonet
Subject: [Histonet] Fite stain


Good morning everyone,

I am having a problem with my Fite stains.  Most stain beautifully but I
have had trouble with a few cases.  Here are the doc's comments:

"case xxxx has some lipomembranous change- and this may not really be an
entirely false positive result. But there is clearly no explanation for
the stuff in xxxx that stains, including some fibrin. I've never seen
this before. It's not the usual round spots from inadequately rinsing
off the peanut oil...."

"A couple of days ago, I saw a granulomatous reaction that had tiny Fite
positive "blobbos" that looked like microbes in tiny cystic spaces
within the granulomas. I initially thought they might be real organisms
but then I did a TB stain and proved that they were nothing. Just
moments ago, XXXX had a case that was essentially identical. The Fite
stain is working perfectly for ID of microbes, but it seems to have a
false positive kind of reactivity that can be seen in small spaces
within a granulomatous infiltrate. On an occasional basis only."

Does anyone have any idea?  Our procedure is 2 changes of xylene/peanut
oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in
1% acid alcohol, rinse, counterstain in methylene blue, air dry.

Thanks in advance,


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