[Histonet] Fite stain

[Tony Henwood]

No,

Sulphuric acid does not differentiate as well as acid alcohol. Leprosy
bacilli do not hang on to the carbol fuchsin as well as other
mycobacterium so that is one reason why low pH water (ie 1% sulphuric
acid) is preferred over acid alcohol.

One of the other attributes of the Fite modification is the use of a
vegetable oil in the dewaxing step. It is believed that the oil helps
the leprae bacilli cell wall better retain the carbol fuchsin dye.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Martin, Erin [mailto:Erin.Martin <@t> ucsf.edu] 
Sent: Thursday, 12 June 2008 11:09 PM
To: Rena Fail; Tony Henwood; histonet
Subject: RE: [Histonet] Fite stain


I am using the American MasterTech kit and following their procedure...
Do you think that changing to a sulfuric acid differentiation would
remove the false postives in granulomatous infiltrate that the acid
alcohol is apparently not removing?
 
Thanks
Erin

________________________________

From: Rena Fail [mailto:renafail <@t> bellsouth.net]
Sent: Sat 7/12/2008 4:39 PM
To: 'Tony Henwood'; Martin, Erin; 'histonet'
Subject: RE: [Histonet] Fite stain



Acid alcohol will remove your dye from those bugs requiring the peanut
oil (usually Lepra Bacilli). It will also remove it from other acid fast
organisms, but at a slower rate. 1% sulfuric acid differentiation gives
more consistent results. Rena Fail

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: Wednesday, June 11, 2008 7:20 PM
To: Martin, Erin; histonet
Subject: RE: [Histonet] Fite stain

Your differentiation step is used in the classic ZN not a Fite. This
might just be a typo but shouldn't it be 1-3% sulphuric acid (or other
acid). The bugs you are trying to demonstrate with the Fite stain are
acid fast but acid-alcohol labile.

This won't solve the problem with you odd staining though

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist The Children's Hospital at Westmead, Locked
Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Erin
Sent: Wednesday, 11 June 2008 10:57 PM
To: histonet
Subject: [Histonet] Fite stain


Good morning everyone, 

I am having a problem with my Fite stains.  Most stain beautifully but I
have had trouble with a few cases.  Here are the doc's comments:

"case xxxx has some lipomembranous change- and this may not really be an
entirely false positive result. But there is clearly no explanation for
the stuff in xxxx that stains, including some fibrin. I've never seen
this before. It's not the usual round spots from inadequately rinsing
off the peanut oil...."

"A couple of days ago, I saw a granulomatous reaction that had tiny Fite
positive "blobbos" that looked like microbes in tiny cystic spaces
within the granulomas. I initially thought they might be real organisms
but then I did a TB stain and proved that they were nothing. Just
moments ago, XXXX had a case that was essentially identical. The Fite
stain is working perfectly for ID of microbes, but it seems to have a
false positive kind of reactivity that can be seen in small spaces
within a granulomatous infiltrate. On an occasional basis only."

Does anyone have any idea?  Our procedure is 2 changes of xylene/peanut
oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in
1% acid alcohol, rinse, counterstain in methylene blue, air dry.

Thanks in advance,


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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

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virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
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