[Histonet] Decolorize PAS

Christine Bark CBark <@t> memorialcare.org
Thu Jun 5 16:30:53 CDT 2008

Hello all,
            My pathologist asked me to de-stain a PAS-stained section of
liver and re-stain it with a trichrome. I've never decolorized anything
other than an H&E. Would I still use acid alcohol and if so for how
long? I've read that potassium metabisulfite will take out excess
Schiff's but I've never tried that and I don't have any. Any
Thank you,
Christine Bark HT(ASCP)

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