[Histonet] Decolorize PAS

[Rene J Buesa]

PAS is a very strong reaction extremely difficult to destain. Acid treatments that could fade it will affect the reactivity of the tissue for further staining procedures.
  René J.

Christine Bark <CBark <@t> memorialcare.org> wrote:
  Hello all,
My pathologist asked me to de-stain a PAS-stained section of
liver and re-stain it with a trichrome. I've never decolorized anything
other than an H&E. Would I still use acid alcohol and if so for how
long? I've read that potassium metabisulfite will take out excess
Schiff's but I've never tried that and I don't have any. Any
suggestions/tips? 
Thank you,

Christine Bark HT(ASCP)

______________________________________________________________________________
Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or distribution 
is prohibited. If you are not the intended recipient, please contact the sender 
by reply e-mail and destroy all copies of the original message.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet