[Histonet] Decolorize PAS

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Jun 6 08:03:46 CDT 2008

PAS is a very strong reaction extremely difficult to destain. Acid treatments that could fade it will affect the reactivity of the tissue for further staining procedures.
  René J.

Christine Bark <CBark <@t> memorialcare.org> wrote:
  Hello all,
My pathologist asked me to de-stain a PAS-stained section of
liver and re-stain it with a trichrome. I've never decolorized anything
other than an H&E. Would I still use acid alcohol and if so for how
long? I've read that potassium metabisulfite will take out excess
Schiff's but I've never tried that and I don't have any. Any
Thank you,

Christine Bark HT(ASCP)

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