[Histonet] plastic sectioning for light microscopy-HELP!!

[Akemi Allison-Tacha]

Hi Danielle,
Way back in my distant past I did GMA for OHSU
Surgical Path Dept.  We would put a few drops of
acetone in our water.  The section would spin a little
on the water then settle.  This helped to remove
wrinkles.  We would then place the slide on a slide
warmer to further smooth out wrinkles and adhere the
section.

Hope this helps.

Akemi Allison-Tacha BS, HT (ASCP) HTL
Client Services Manager
PhenoPath Laboratories
551 N 34th St., #100
Seattle, WA 98103
(206) 374-9000
akemi <@t> phenopath.com 
http://www.phenopath.com
--- Danielle Crippen <dcrippen <@t> buckinstitute.org>
wrote:

> Dear Histo-experts,
> 
>  
> 
> This is a first for me as I have only done ultrathin
> sectioning for EM.
> 
> 
>  
> 
> I need 0.5-1um serial sections for light microscopy.
>  My main problem at
> this point is transferring the ribbons to a glass
> (plus) slide.  No
> matter how I try to attempt the transfer, the ribbon
> comes apart and/or
> sections are lost or badly wrinkled.  Any and all
> suggestions in this
> area are very welcome!!
> 
>  
> 
> Additionally...once I actually get the sections onto
> the slide (which
> feels like it might be cause for a celebration at
> this point!!)...what
> are the most recommended H&E staining methods (these
> samples are
> embedded in EPON)?
> 
>  
> 
> Again...I am a complete novice at plastic sectioning
> for light
> microscopy...so any suggestions are welcome...even
> the most trivial:-)
> 
>  
> 
> A thousand thanks in advance!!
> 
>  
> 
>  
> 
>  
> 
> danielle
> 
>  
> 
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