[Histonet] Osteoblast and osteoclast staining

Geoff McAuliffe mcauliff <@t> umdnj.edu
Wed Jan 23 11:41:39 CST 2008

Osteoclasts are easy to recognize in H&E staining, they are large 
multinucleate cells almost always found in depressions (Howship's 
lacuna) on the surface of bone. No other bone cell looks like an osteoclast.
Osteoblasts are found on the surface of bone, any surface not just the 
outer surface. Osteocytes are found encased in bone matrix.


Majid ghoddusi wrote:
> Hello all,
> I have some paraffin-embedded Murine bone that have been decalcified with a
> hydrochloric acid-based decalcifying solution. I would like to stain the
> osteoblasts and osteoclasts in this tissue if possible. What are my options?
> The information that I am getting suggests that my chances of a successful
> staining with TRAP is somewhat limited since the tissue has been decalcified
> in this manner. I would appreciate any help I can get form the list.
> Thank you,
> Majid
> *Majid Ghoddusi, DVM, PhD*
> *Veterinary Pathologist*
> *Comparative Biosciences, Inc.*
> *http://www.compbio.com/* <http://www.compbio.com/>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu

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