[Histonet] Sections falling off again

MVaughan4 <@t> ucok.edu MVaughan4 <@t> ucok.edu
Wed Jan 23 12:49:54 CST 2008

I section skin and skin equivalents (artificial skin). I have antigens 
that require citrate buffer or protease XXIV/XXV retrieval. I often have 
trouble keeping the sections on the slides, whether I use Fisher  or VWR 
superfrost plus or ProbeOn plus. Maybe they are not sticking initially. I 
have a water bath set at 42degreesC, the sections are about 8 microns, I 
put the slides overnight in a 37degree oven before refrigerating them. 
Does anybody see anything wrong with this? Thanks for your help, my 
students and I will be glad to get this fixed.
Melville B. Vaughan, Ph. D.
Assistant Professor of Biology
Campus Coordinator, NSF Sure-Step program
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034

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