[Histonet] Osteoblast and osteoclast staining

[Majid ghoddusi]

Hello all,

I have some paraffin-embedded Murine bone that have been decalcified with a
hydrochloric acid-based decalcifying solution. I would like to stain the
osteoblasts and osteoclasts in this tissue if possible. What are my options?
The information that I am getting suggests that my chances of a successful
staining with TRAP is somewhat limited since the tissue has been decalcified
in this manner. I would appreciate any help I can get form the list.

Thank you,
Majid



*Majid Ghoddusi, DVM, PhD*
*Veterinary Pathologist*
*Comparative Biosciences, Inc.*
*http://www.compbio.com/* <http://www.compbio.com/>