AW: [Histonet] hb crystals

[Jennifer MacDonald]

Formalin pigment can be confirmed by removing it.  You can use alcoholic 
picric acid or alkaline alcohol. 

Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu



Paul Bradbury <histology.bc <@t> shaw.ca> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/28/2008 04:27 PM

To
gu.lang <@t> gmx.at, HistoNet Server <histonet <@t> pathology.swmed.edu>
cc

Subject
Re: AW: [Histonet] hb crystals






I have never seen the PAS reaction give positive results with formalin 
pigment. Even if it did react, how would it be seen. The pigment is 
already dark brown-black, so colouring it red would have no visible 
effect. The same problem applies to any silver methods which might work 
... it's already almost black, so making it a "darker black" would not 
be much help. The only definitive way to identify it is to use its 
birefringent properties.

Paul
Kamloops, Canada


Gudrun Lang wrote:
> If this crystals are formalin-pigment they have reducing capacitiy.
> Therefore with silverimpregnation techniques like Masson this would 
render
> black spots. Also the PAS stain would give positivity.
>
> Gudrun Lang
> 
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Jim
> Manavis
> Gesendet: Donnerstag, 28. Februar 2008 03:41
> An: Histonet
> Betreff: [Histonet] hb crystals
>
> Dear Sir or Madam:
>
> 
>
> I am a PhD student studying intracerebral haemorrhage in the rat and 
have
> found numerous crystals contained within the haemorrhage, which I 
presume
> are haemoglobin crystals, although it would be nice to demonstrate this.
> Pearl's stain doesn't work, presumably because the iron is ferrous and 
bound
> tightly, and Lillie's method with ferricyanide and HCL likewise doesn't
> work, presumably because the iron is bound. Our standard DAB technique 
for
> immunohistochemistry doesn't seem to react either. How might I best
> determine whether or not the crystals are haemoglobin crystals?
>
> 
>
> Yours Sincerely, 
>
> 
>
> Tim Kleinig
>
> 
>
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