[Histonet] formaldehyd fixation - ph

[Gudrun Lang]

Hi dear listmembers,

Is it generally accepted, that formaldehyd fixation in an acid environment
is less effective than in basic? Or in other words: Does formaldehyde bind
to proteins in acid solution to a smaller amount than in basic solutions?

 

I have found a publication (Theis, 1944) where the the amount of bound
formaldehyde was plotted against pH. And the line increased with the pH. A
few years later Gustavson published that Theis' methods of measurement were
wrong and also his findings about the reactionpartners of formaldehyde. But
I cannot find a newer paper about the effect of pH on formaldehydfixation.

Can anyone help me out?

 

Froehliche Weihnachten!

Gudrun Lang