[Histonet] formaldehyd fixation - ph
gu.lang <@t> gmx.at
Tue Dec 23 05:56:23 CST 2008
Hi dear listmembers,
Is it generally accepted, that formaldehyd fixation in an acid environment
is less effective than in basic? Or in other words: Does formaldehyde bind
to proteins in acid solution to a smaller amount than in basic solutions?
I have found a publication (Theis, 1944) where the the amount of bound
formaldehyde was plotted against pH. And the line increased with the pH. A
few years later Gustavson published that Theis' methods of measurement were
wrong and also his findings about the reactionpartners of formaldehyde. But
I cannot find a newer paper about the effect of pH on formaldehydfixation.
Can anyone help me out?
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