Re: RE: [Histonet] stickest slides for brain sections or how tocoat aproper slide for urself

[tf]

Thanks for sharing.
I do not have big problem in mounted slides from microtome - you pick the sections up from solution. 
But I did have some sections prepared in cryostat came off during BrdU IHC (citrate buffer 95 degree for 30 min , so harsh~~). I am not sure whether this is because of embedding (O.C.T) ? Or something else. Even you use pre-coated slides. 

And, As I mentioned in last email, we found the 0.25% gel-slide works as good as pre-coated slides if the sections were mounted from PBS, washed out all embedding materials. 


2008-12-17 



tf 



发件人： Mejia, Maria 
发送时间： 2008-12-17  01:24:31 
收件人： tifei; Alexandra Meinl; cmmathis1 
抄送： histonet 
主题： RE: [Histonet] stickest slides for brain sections or how tocoat aproper slide for urself 
 
This is for anyone's interest who work with 40um primate brain section 2x3 inches. We routinely 
mount our IHC stained sections from working PBS on pre-subbed brain slides from Brain Research 
Labs.
To keep the sections on the slides we use a very fine (#0) brush to brush the section flat on the
slide & to remove any PBS that may get trap under the section. We dry overnight. Next day, I
put in oven at 37C - 30 minutes to further dry.
Regards 
Maria Bartola Mejia
Histology Manager
Department of Neurosurgery
UCSF
San Francisco, CA 94103
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of tf
Sent: Tue 12/16/2008 9:00 AM
To: Alexandra Meinl; cmmathis1
Cc: histonet
Subject: [Histonet] stickest slides for brain sections or how to coat aproper slide for urself

I have similar questions...
for brain sections.
we found that 0.25% gel coated slides are better than 0.5% or 1% gel in sticking 40 um brain sections.
just want to share this with you guys.
2008-12-17 
tf 
???: Alexandra Meinl 
????: 2008-12-17  00:27:04 
???: cmmathis1 
??: histonet 
??: Re: [Histonet] What is the stickest slides 

I like Roti-Bond Adhesion slides for difficult material like bone, you can
also use HIER without problems. But these slides are quite expensive and
require 2-3 days drying time.
Alex
2008/12/16 <cmmathis1 <@t> bellsouth.net>
> Hello Histonetters,
> We are having trouble getting our paraffin processed cow meniscus sections
> (at 5 microns) to stay on our Superfrost Plus slides.  We are drying them
> overnight in a 58 C oven.  The H&Es look great but the immunos wash every
> time.
> What is your favorite slide for these hard to stay on samples?
> Thanks for your time,
> Cathy
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