[Histonet] stickest slides for brain sections or how to coat aproper slide for urself

[Mejia, Maria]

This is for anyone's interest who work with 40um primate brain section 2x3 inches. We routinely 
mount our IHC stained sections from working PBS on pre-subbed brain slides from Brain Research 
Labs.

To keep the sections on the slides we use a very fine (#0) brush to brush the section flat on the
slide & to remove any PBS that may get trap under the section. We dry overnight. Next day, I
put in oven at 37C - 30 minutes to further dry.

Regards 
Maria Bartola Mejia
Histology Manager
Department of Neurosurgery
UCSF
San Francisco, CA 94103


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of tf
Sent: Tue 12/16/2008 9:00 AM
To: Alexandra Meinl; cmmathis1
Cc: histonet
Subject: [Histonet] stickest slides for brain sections or how to coat aproper slide for urself
 
I have similar questions...
for brain sections.

we found that 0.25% gel coated slides are better than 0.5% or 1% gel in sticking 40 um brain sections.

just want to share this with you guys.


2008-12-17 



tf 



???: Alexandra Meinl 
????: 2008-12-17  00:27:04 
???: cmmathis1 
??: histonet 
??: Re: [Histonet] What is the stickest slides 
 
I like Roti-Bond Adhesion slides for difficult material like bone, you can
also use HIER without problems. But these slides are quite expensive and
require 2-3 days drying time.
Alex
2008/12/16 <cmmathis1 <@t> bellsouth.net>
> Hello Histonetters,
> We are having trouble getting our paraffin processed cow meniscus sections
> (at 5 microns) to stay on our Superfrost Plus slides.  We are drying them
> overnight in a 58 C oven.  The H&Es look great but the immunos wash every
> time.
> What is your favorite slide for these hard to stay on samples?
> Thanks for your time,
> Cathy
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