[Histonet] stickest slides for brain sections or how to coat a proper slide for urself

[tf]

I have similar questions...
for brain sections.

we found that 0.25% gel coated slides are better than 0.5% or 1% gel in sticking 40 um brain sections.

just want to share this with you guys.


2008-12-17 



tf 



发件人： Alexandra Meinl 
发送时间： 2008-12-17  00:27:04 
收件人： cmmathis1 
抄送： histonet 
主题： Re: [Histonet] What is the stickest slides 
 
I like Roti-Bond Adhesion slides for difficult material like bone, you can
also use HIER without problems. But these slides are quite expensive and
require 2-3 days drying time.
Alex
2008/12/16 <cmmathis1 <@t> bellsouth.net>
> Hello Histonetters,
> We are having trouble getting our paraffin processed cow meniscus sections
> (at 5 microns) to stay on our Superfrost Plus slides.  We are drying them
> overnight in a 58 C oven.  The H&Es look great but the immunos wash every
> time.
> What is your favorite slide for these hard to stay on samples?
> Thanks for your time,
> Cathy
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