[Histonet] Re: PCNA

[Hobbs, Carl]

Use a PC10 mouse clone for pwax sections: works very well on many species that have been fixed in routine Formalin.
Imho, no special procedures reqd.
Sure, AR is best.
If you wish to check out this site: http://www.immunoportal.com/index.php
you might find further help?
Also , be very careful if you use PCNA as a proliferation marker......it has a very long half-life compared with Ki67.
So, use an anti Ki67 Ab instead for general proliferation studies that cannot include use of BrdU.
Sure, if you want to assess a Mitotic index...use pH3 Ab on pwax sections.
carl