[Histonet] Re: processing mouse seminal vesicles

[Susan Raibley]

Try this processing schudule for mouse tissue:
 
70% 15 min
95% 15 min
95% 15 min
100% 15 min
100% 15 min
100% 15 min
100% 15 min
Xylene 15 min
Xylene 15 min
Paraffin 15 min
Paraffin 15 min
Paraffin 15 min
Paraffin 15 min
 
Good luck!
Susan Bincsik
 
 
 
 
Does anybody have a protocol for this?  My last batch of these came out VERY dry and crunchy when run with other tissues on my standard protocol, which is as follows:
(They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before processing.)

70%: 30 min
80%: 30 min
95%: 45 min
95%: 45 min
100%: 45 min
100%: 45 min
xylene: 45 min
xylene: 45 min
Paraffin: 30 min
Paraffin: 30min
Paraffin: 30 min

My other thought is that something is up with our VIP 5 processor, though no error messages are showing up.  Any and all suggestions are most welcome.

Thanks in advance,
Kathleen Roberts
Rutgers University