[Histonet] de-waxing / staining station question

Geoff McAuliffe mcauliff <@t> umdnj.edu
Wed Dec 10 15:12:48 CST 2008

Two changes of xylene is a minimum. Also, almost every time someone asks 
about uneven staining it is due to inadequate removal of wax.


Anna K. Schultz wrote:
> Hi all,
>  In an effort to reduce space usage, it has been suggested that I 
> eliminate one of two xylene steps (and lengthen the time in the 
> remaining xylene) when de-waxing paraffin sections.  I do not see any 
> protocols that only use one step of xylene.  Are two strictly 
> necessary?  I would like to know the reasoning.
>  Also, do gill hematoxylins and alcoholic eosin need to be used and 
> stored under a hood?  I've read the MSDS but it wasn't entirely clear 
> if they should just be used with caution or strictly under the hood.  
> They are in staining buckets with lids (the lids are sometimes leaky).
>  I thought I would seek some advice here.
> Thanks,
> Anna
> basic science research core tech
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu

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