[Histonet] Processing mouse seminal vesicles-thanks!

[Kathleen Roberts]

Well, I have a number of suggestions now; once I get past the holidays 
I'll start trying them out when the next batch of samples comes in.  We 
provide histology and pathology services for our dept and university, so 
it's all animal tissue.

Thanks again to everybody, and I'll let you know how it turns out.

-Kathleen
Rutgers University

Rene J Buesa wrote:

> Mouse tissues, any one, will dry out too much with ethanol and xylene.
> I advise you to stop using ethanol and xylene and substitute BOTH with 
> iso-propanol at the concentrations and times you are using now.
> Instead of the first paraffin, use a mixture 1:1 of iso-propanol and 
> paraffin, followed by the 2 paraffins.
> Try it and you will notice the difference (and the saings).
> René J.
>
> --- On Wed, 12/10/08, Kathleen Roberts <kgrobert <@t> rci.rutgers.edu> wrote:
>
>     From: Kathleen Roberts <kgrobert <@t> rci.rutgers.edu>
>     Subject: [Histonet] Processing mouse seminal vesicles
>     To: "'histonet <@t> lists.utsouthwestern.edu'"
>     <histonet <@t> lists.utsouthwestern.edu>
>     Date: Wednesday, December 10, 2008, 11:54 AM
>
>Does anybody have a protocol for this?  My last batch of these came out VERY dry
>and crunchy when run with other tissues on my standard protocol, which is as
>follows:
>(They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before
>processing.)
>
>70%: 30 min
>80%: 30 min
>95%: 45 min
>95%: 45 min
>100%: 45 min
>100%: 45 min
>xylene: 45 min
>xylene: 45 min
>Paraffin: 30 min
>Paraffin: 30min
>Paraffin: 30 min
>
>My other thought is that something is up with our VIP 5 processor, though no
>error messages are showing up.  Any and all suggestions are most welcome.
>
>Thanks in advance,
>Kathleen Roberts
>Rutgers University
>
>
>
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