[Histonet] Re: Blue Haze

[Jennifer Springsteen]

I also use the Surgipath SelecTech H&E system and had to solve the blue haze
problem in our lab.  We've had great success simply using warm tap water to
wash the slides after hematoxylin, rather than cold tap water.  Hope that
helps!

-- 
Jennifer L. Springsteen, B.S.
Lab Manager, Assistant Scientist
Lillehei Heart Institute Histology & Microscopy Core Facility
University of Minnesota School of Medicine, Division of Cardiology
312 Church Street SE
4-266 Nils Hasselmo Hall
Minneapolis, MN  55455
Lab: 612-626-3090
Cell: 651-357-7916
Fax: 612-624-8118

> Date: Wed, 3 Dec 2008 12:47:34 -0500
> From: "Marshall, Kimberly" <Kimberly.Marshall <@t> ahss.org>
> Subject: [Histonet] Blue haze
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <C1A04A53C51BE149AA98408B31BC3045030E80BE <@t> LKMCXCH13.ADVENTISTCORP.NET>
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> 
> Howdy all,
>  
>    I have just changed over to Surgi Path's H & E system.  With the latest
> fear of running out of hematoxylin and all my Pathologist wanted us to change
> over.  Its a great stain but we have a bad "haze"  in the background.  It is
> not hurting the tissue or DX at all, but to ME it looks awful.  Is there
> anyone else out there using this?   We are not using positive charged slides
> or anything added to my water bath.  Any suggestions????  Thanks in advance
>  
> Kimberly Marshall HT (ASCP)