[Histonet] What is the name of this blue stain?

renafail <@t> bellsouth.net renafail <@t> bellsouth.net
Wed Dec 3 13:27:47 CST 2008


 
 Monastral fast blue, an insoluble pigment
Rena Fail
 -------------- Original message from "Madary, Joseph" <MadaryJ <@t> MedImmune.com>: --------------

 Alcian Blue goes into an alkaline alcohol for one hour as part of the old style movat.  What is the final name of that blue color?  Trying to train someone and 
> could not find it.
> 
> 
> Nick Madary, HT/HTL(ASCP)QIHC
> Medimmune Histology Laboratory Mgr
> One Medimmune Way, Lab 2438-Area 4
> Gaithersburg, MD 20878
> 
> ph 301.398.4745/6360
> fx  301.398.9745
> To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for 
> any purpose. Thank you for your cooperation.
> >P Please consider the environment before printing this e-mail
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> histonet-request <@t> lists.utsouthwestern.edu
> Sent: Wednesday, December 03, 2008 1:04 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 61, Issue 5
> 
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> Today's Topics:
> 
>    1. 2x3 slides (Pat Keller)
>    2. Re: 2x3 slides (Pamela Marcum)
>    3. lids for stainer containers (Perry, Margaret)
>    4. RE: RE: Flame substitute for embedding (Bernice Frederick)
>    5. Blue haze (Marshall, Kimberly)
>    6. Re: PLP fixative lysine  (Gayle Callis)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Wed, 03 Dec 2008 11:29:55 -0600
> From: Pat Keller 
> Subject: [Histonet] 2x3 slides
> To: 
> Message-ID: 
> Content-Type: text/plain;	charset="US-ASCII"
> 
> Fisher has them
> 
> 
> MILLIPORE CORP  MICROSCOPE SLIDES 2"X3" 72/PK
> 
> Fisher offers many products that do not appear in our catalogs. This may be one 
> of those products, so pictures and detailed descriptions are not available. 
> However, you may be able to order it by adding it to your shopping cart.
>  
> 
> Item
> Description     Catalog Number     Quantity     Price
> MICROSCOPE SLIDES 2"X3" 72/PK
>     XX1007615
> Vendor No.:XX1007615         Pack of 72 for $51.26
> 
> 
> Patricia Keller, 
> Sr. Res. Tech.   Histology Core Manager
> Washington University School of Medicine Department of Otolaryngology Campus box 
> 8115
> 4566 Scott Ave.
> St. Louis,   MO.   63110
> KellerP <@t> ent.wustl.edu
> http://www.otocore.org/
> 
> OFFICE    314-747-7166
> FAX          314-747-7230
> 
> 
> 
The materials in this message are private and may contain Protected 
> Healthcare Information. If you are not the intended recipient, be advised that 
> any unauthorized use, disclosure, copying or the taking of any action in 
> reliance on the contents of this information is strictly prohibited. If you have 
> received this email in error, please immediately notify the sender via telephone 
> or return mail.
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Wed, 3 Dec 2008 17:34:48 +0000 (UTC)
> From: Pamela Marcum 
> Subject: Re: [Histonet] 2x3 slides
> To: Pat Keller 
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	
> <966250373.78611228325688567.JavaMail.root <@t> sz0001a.westchester.pa.mail.comcast.n
> et>
> 	
> Content-Type: text/plain; charset=utf-8
> 
> 
> 
> 
> I got the slides from Thermo Fisher with some problems a long while ago.  The 
> issue was finding a 100 slide box for storage and shipping.  I did order them 
> this morning from Brain Research and they had stock of the boxes.  Thanks to 
> everyone who helped and now I know where to go for the boxes next time. 
> 
> 
> 
> Pam Marcum 
> 
> 
> ----- Original Message -----
> From: "Pat Keller" 
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Wednesday, December 3, 2008 12:29:55 PM GMT -05:00 US/Canada Eastern
> Subject: [Histonet] 2x3 slides 
> 
> Fisher has them 
> 
> 
> MILLIPORE CORP  MICROSCOPE SLIDES 2"X3" 72/PK 
> 
> Fisher offers many products that do not appear in our catalogs. This may be one 
> of those products, so pictures and detailed descriptions are not available. 
> However, you may be able to order it by adding it to your shopping cart. 
>   
> 
> Item
> Description     Catalog Number     Quantity     Price MICROSCOPE SLIDES 
> 2"X3" 72/PK     XX1007615 Vendor No.:XX1007615         Pack of 72 for 
> $51.26 
> 
> 
> Patricia Keller,
> Sr. Res. Tech.   Histology Core Manager Washington University School of 
> Medicine Department of Otolaryngology Campus box 8115
> 4566 Scott Ave. 
> St. Louis,   MO.   63110
> KellerP <@t> ent.wustl.edu
> http://www.otocore.org/ 
> 
> OFFICE    314-747-7166
> FAX          314-747-7230 
> 
> 
> 
The materials in this message are private and may contain Protected 
> Healthcare Information. If you are not the intended recipient, be advised that 
> any unauthorized use, disclosure, copying or the taking of any action in 
> reliance on the contents of this information is strictly prohibited. If you have 
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> 
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> 
> ------------------------------
> 
> Message: 3
> Date: Wed, 3 Dec 2008 11:46:33 -0600
> From: "Perry, Margaret" 
> Subject: [Histonet] lids for stainer containers
> To: "histonet <@t> lists.utsouthwestern.edu"
> 	
> Message-ID:
> 	
> Content-Type: text/plain; charset="us-ascii"
> 
> We are looking for a source that sells a lid that looks like wax covered 
> cardboard.  We use them on top of the Shandon slide containers holding xylene or 
> formula 83 while waiting to coverslip.  They are about 5x7 and very thin.  They 
> have been here for years so I have no idea where they came from.  Any help in 
> finding them or a substitute will be greatly appreciated.  Thanks.
> 
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Wed, 3 Dec 2008 11:48:12 -0600
> From: "Bernice Frederick" 
> Subject: RE: [Histonet] RE: Flame substitute for embedding
> To: "'Jacqueline Farnsworth'" ,
> 	"'Celebre Julia'" ,
> 	
> Message-ID: <000001c9556f$52b14590$d00f7ca5 <@t> lurie.northwestern.edu>
> Content-Type: text/plain;	charset="us-ascii"
> 
> We have a Shandon Paratrimmer, which melts the wax not only off blocks but
> does a good job of keeping those forceps clean and hot.
> We have a bacti-incinerator, but know we can get a tall flame if we leave
> the paraffin coated forceps in there!!
> Bernice
> 
> 
> Bernice Frederick HTL (ASCP)
> Northwestern University
> Pathology Core Facility
> ECOGPCO-RL 
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jacqueline
> Farnsworth
> Sent: Wednesday, December 03, 2008 9:44 AM
> To: Celebre Julia; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Flame substitute for embedding
> 
> We also used to have an open flame while embedding!  Now, we simply have a
> policy in place where after EACH cassette is embedded, the Techs MUST
> thoroughly wipe their forceps with disposable Kleenex (and then dispose of
> it!). Also, we use toothless forceps as much as possible to reduce the
> possibility of cross contamination.
> 
> 
> Jacqueline Farnsworth
> Anatomic Pathology, Tech III
> Foothills Medical Centre
> Calgary Laboratory Services
> 
> Ph: 403-944-1578
> Fax: 403-944-4748
> P Please consider the environment before printing this email.
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre Julia
> [celebrej <@t> HHSC.CA]
> Sent: December 3, 2008 8:38 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Flame substitute for embedding
> 
> Hello Histoland!!
> 
> We have just been told  we are no longer allowed to use alcohol burners
> while embedding and we are to find a safer substitute, something to do with
> no liking an open flame in the lab.  Other than incinerators or 6 pairs of
> forceps at each centre, we've run out of ideas so we are asking all you
> experts out there what your current practice is.. oh.. this change needs to
> be done by this Friday!!
> 
> Julia Celebre MLT
> Anatomic Pathology
> Hamilton General Hospital
> 905-527-0271  ext 46179
> email: celebrej <@t> hhsc.ca
> 
> 
> 
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> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Wed, 3 Dec 2008 12:47:34 -0500
> From: "Marshall, Kimberly" 
> Subject: [Histonet] Blue haze
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	
> Content-Type: text/plain; charset=iso-8859-1
> 
> Howdy all,
>  
>    I have just changed over to Surgi Path's H & E system.  With the latest fear 
> of running out of hematoxylin and all my Pathologist wanted us to change over.  
> Its a great stain but we have a bad "haze"  in the background.  It is not 
> hurting the tissue or DX at all, but to ME it looks awful.  Is there anyone else 
> out there using this?   We are not using positive charged slides or anything 
> added to my water bath.  Any suggestions????  Thanks in advance
>  
> Kimberly Marshall HT (ASCP)
> 
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> 
> ------------------------------
> 
> Message: 6
> Date: Wed, 3 Dec 2008 10:56:21 -0700
> From: "Gayle Callis" 
> Subject: [Histonet] Re: PLP fixative lysine 
> To: "Histonet" 
> Message-ID: 
> Content-Type: text/plain;	charset="iso-8859-1"
> 
> For the PLP fixative, the full name for the lysine is L- lysine 
> monohydrochloride.  Sigma cat# L5626. 
> 
> Gayle M. Callis
> HTL(ASCP)HT,MT
> 
> ------------------------------
> 
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> End of Histonet Digest, Vol 61, Issue 5
> ***************************************
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> To the extent this electronic communication or any of its attachments contain 
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> have received this electronic communication in error, please reply to the sender 
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