[Histonet] What is the name of this blue stain?

Wed Dec 3 13:55:38 CST 2008

monastral fast blue

"Madary, Joseph" <MadaryJ <@t> MedImmune.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/03/2008 11:15 AM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] What is the name of this blue stain?

 Alcian Blue goes into an alkaline alcohol for one hour as part of the old 
style movat.  What is the final name of that blue color?  Trying to train 
someone and could not find it.

Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory Mgr
One Medimmune Way, Lab 2438-Area 4
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

To the extent this electronic communication or any of its attachments 
contain information that is not in the public domain, such information is 
considered by MedImmune to be confidential and proprietary, and expected 
to be used only by the individual(s) for whom it is intended. If you have 
received this electronic communication in error, please reply to the 
sender advising of the error in transmission and delete the original 
message and any accompanying documents from your system immediately, 
without copying, reviewing or otherwise using them for any purpose. Thank 
you for your cooperation.
>P Please consider the environment before printing this e-mail

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 03, 2008 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 61, Issue 5

Send Histonet mailing list submissions to
                 histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
                 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
                 histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
                 histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than 
"Re: Contents of Histonet digest..."

Today's Topics:

   1. 2x3 slides (Pat Keller)
   2. Re: 2x3 slides (Pamela Marcum)
   3. lids for stainer containers (Perry, Margaret)
   4. RE: RE: Flame substitute for embedding (Bernice Frederick)
   5. Blue haze (Marshall, Kimberly)
   6. Re: PLP fixative lysine  (Gayle Callis)

----------------------------------------------------------------------

Message: 1
Date: Wed, 03 Dec 2008 11:29:55 -0600
From: Pat Keller <kellerp <@t> ent.wustl.edu>
Subject: [Histonet] 2x3 slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C55C1E33.50C1%kellerp <@t> ent.wustl.edu>
Content-Type: text/plain;                charset="US-ASCII"

Fisher has them

MILLIPORE CORP  MICROSCOPE SLIDES 2"X3" 72/PK

Fisher offers many products that do not appear in our catalogs. This may 
be one of those products, so pictures and detailed descriptions are not 
available. However, you may be able to order it by adding it to your 
shopping cart.

Item
Description     Catalog Number     Quantity     Price
MICROSCOPE SLIDES 2"X3" 72/PK
    XX1007615
Vendor No.:XX1007615         Pack of 72 for $51.26

Patricia Keller, 
Sr. Res. Tech.   Histology Core Manager
Washington University School of Medicine Department of Otolaryngology 
Campus box 8115
4566 Scott Ave.
St. Louis,   MO.   63110
KellerP <@t> ent.wustl.edu
http://www.otocore.org/

OFFICE    314-747-7166
FAX          314-747-7230

<br/>The materials in this message are private and may contain Protected 
Healthcare Information. If you are not the intended recipient, be advised 
that any unauthorized use, disclosure, copying or the taking of any action 
in reliance on the contents of this information is strictly prohibited. If 
you have received this email in error, please immediately notify the 
sender via telephone or return mail.

------------------------------

Message: 2
Date: Wed, 3 Dec 2008 17:34:48 +0000 (UTC)
From: Pamela Marcum <mucram11 <@t> comcast.net>
Subject: Re: [Histonet] 2x3 slides
To: Pat Keller <kellerp <@t> ent.wustl.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:

<966250373.78611228325688567.JavaMail.root <@t> sz0001a.westchester.pa.mail.comcast.net>

Content-Type: text/plain; charset=utf-8

I got the slides from Thermo Fisher with some problems a long while ago.Â  
The issue was finding a 100 slide box for storage and shipping.Â  I did 
order them this morning from Brain Research and they had stock of the 
boxes.Â  Thanks to everyone who helped and now I know where to go for the 
boxes next time. 

Pam Marcum 

----- Original Message -----
From: "Pat Keller" <kellerp <@t> ent.wustl.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 3, 2008 12:29:55 PM GMT -05:00 US/Canada Eastern
Subject: [Histonet] 2x3 slides 

Fisher has them 

MILLIPORE CORP Â MICROSCOPE SLIDES 2"X3" 72/PK 

Fisher offers many products that do not appear in our catalogs. This may 
be one of those products, so pictures and detailed descriptions are not 
available. However, you may be able to order it by adding it to your 
shopping cart. 
Â  

Item
Description Â  Â  Catalog Number Â  Â  Quantity Â  Â  Price MICROSCOPE 
SLIDES 2"X3" 72/PK Â Â  Â XX1007615 Vendor No.:XX1007615 Â  Â  Â  Â  Pack 
of 72 for $51.26 

Patricia Keller,
Sr. Res. Tech. Â  Histology Core Manager Washington University School of 
Medicine Department of Otolaryngology Campus box 8115
4566 Scott Ave. 
St. Louis, Â  MO. Â  63110
KellerP <@t> ent.wustl.edu
http://www.otocore.org/ 

OFFICE Â  Â 314-747-7166
FAX Â  Â  Â  Â  Â 314-747-7230 

<br/>The materials in this message are private and may contain Protected 
Healthcare Information. If you are not the intended recipient, be advised 
that any unauthorized use, disclosure, copying or the taking of any action 
in reliance on the contents of this information is strictly prohibited. If 
you have received this email in error, please immediately notify the 
sender via telephone or return mail. 

_______________________________________________ 
Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 3
Date: Wed, 3 Dec 2008 11:46:33 -0600
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] lids for stainer containers
To: "histonet <@t> lists.utsouthwestern.edu"
                 <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
 <FCA5EF47F9BC694CBB4C58FEA042196342F4F11F88 <@t> SDSU-MBX.jacks.local>
Content-Type: text/plain; charset="us-ascii"

We are looking for a source that sells a lid that looks like wax covered 
cardboard.  We use them on top of the Shandon slide containers holding 
xylene or formula 83 while waiting to coverslip.  They are about 5x7 and 
very thin.  They have been here for years so I have no idea where they 
came from.  Any help in finding them or a substitute will be greatly 
appreciated.  Thanks.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

------------------------------

Message: 4
Date: Wed, 3 Dec 2008 11:48:12 -0600
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] RE: Flame substitute for embedding
To: "'Jacqueline Farnsworth'" <Jacqueline.Farnsworth <@t> cls.ab.ca>,
                 "'Celebre Julia'" <celebrej <@t> HHSC.CA>,
                 <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c9556f$52b14590$d00f7ca5 <@t> lurie.northwestern.edu>
Content-Type: text/plain;                charset="us-ascii"

We have a Shandon Paratrimmer, which melts the wax not only off blocks but
does a good job of keeping those forceps clean and hot.
We have a bacti-incinerator, but know we can get a tall flame if we leave
the paraffin coated forceps in there!!
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jacqueline
Farnsworth
Sent: Wednesday, December 03, 2008 9:44 AM
To: Celebre Julia; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Flame substitute for embedding

We also used to have an open flame while embedding!  Now, we simply have a
policy in place where after EACH cassette is embedded, the Techs MUST
thoroughly wipe their forceps with disposable Kleenex (and then dispose of
it!). Also, we use toothless forceps as much as possible to reduce the
possibility of cross contamination.

Jacqueline Farnsworth
Anatomic Pathology, Tech III
Foothills Medical Centre
Calgary Laboratory Services

Ph: 403-944-1578
Fax: 403-944-4748
P Please consider the environment before printing this email.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre Julia
[celebrej <@t> HHSC.CA]
Sent: December 3, 2008 8:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Flame substitute for embedding

Hello Histoland!!

We have just been told  we are no longer allowed to use alcohol burners
while embedding and we are to find a safer substitute, something to do 
with
no liking an open flame in the lab.  Other than incinerators or 6 pairs of
forceps at each centre, we've run out of ideas so we are asking all you
experts out there what your current practice is.. oh.. this change needs 
to
be done by this Friday!!

Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
905-527-0271  ext 46179
email: celebrej <@t> hhsc.ca

This information is directed in confidence solely to the person
named above and may not otherwise be distributed, copied or
disclosed. Therefore, this information should be considered
strictly confidential. If you have received this email in error, please
notify the sender immediately via a return email for further
direction. Thank you for your assistance.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message and any attached documents are only for the use of the 
intended
recipient(s), are confidential and may contain privileged information. Any
unauthorized review, use, retransmission, or other disclosure is strictly
prohibited. If you have received this message in error, please notify the
sender immediately, and then delete the original message. Thank you.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Wed, 3 Dec 2008 12:47:34 -0500
From: "Marshall, Kimberly" <Kimberly.Marshall <@t> ahss.org>
Subject: [Histonet] Blue haze
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
 <C1A04A53C51BE149AA98408B31BC3045030E80BE <@t> LKMCXCH13.ADVENTISTCORP.NET>
Content-Type: text/plain; charset=iso-8859-1

Howdy all,

   I have just changed over to Surgi Path's H & E system.  With the latest 
fear of running out of hematoxylin and all my Pathologist wanted us to 
change over.  Its a great stain but we have a bad "haze"  in the 
background.  It is not hurting the tissue or DX at all, but to ME it looks 
awful.  Is there anyone else out there using this?   We are not using 
positive charged slides or anything added to my water bath.  Any 
suggestions????  Thanks in advance

Kimberly Marshall HT (ASCP)

==============================================================================
The information contained in this message may be privileged and/or 
confidential
and protected from disclosure.  If the reader of this message is not the 
intended 
recipient or an employee or agent responsible for delivering this message 
to the 
intended recipient, you are hereby notified that any dissemination, 
distribution 
or copying of this communication is strictly prohibited.  If you have 
received this
communication in error, please notify the sender immediately by replying 
to this 
message and deleting the material from any computer.

==============================================================================

------------------------------

Message: 6
Date: Wed, 3 Dec 2008 10:56:21 -0700
From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Re: PLP fixative lysine 
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B1D2924BFA1B401FB687B3596A84042E <@t> DHXTS541>
Content-Type: text/plain;                charset="iso-8859-1"

For the PLP fixative, the full name for the lysine is L- lysine 
monohydrochloride.  Sigma cat# L5626. 

Gayle M. Callis
HTL(ASCP)HT,MT

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 61, Issue 5
***************************************

To the extent this electronic communication or any of its attachments 
contain information that is not in the public domain, such information is 
considered by MedImmune to be confidential and proprietary.  This 
communication is expected to be read and/or used only by the individual(s) 
for whom it is intended.  If you have received this electronic 
communication in error, please reply to the sender advising of the error 
in transmission and delete the original message and any accompanying 
documents from your system immediately, without copying, reviewing or 
otherwise using them for any purpose.  Thank you for your cooperation.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet