[Histonet] What is the name of this blue stain?

Madary, Joseph MadaryJ <@t> MedImmune.com
Wed Dec 3 13:09:08 CST 2008 

 Alcian Blue goes into an alkaline alcohol for one hour as part of the old style movat.  What is the final name of that blue color?  Trying to train someone and could not find it.


Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory Mgr
One Medimmune Way, Lab 2438-Area 4
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

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Subject: Histonet Digest, Vol 61, Issue 5

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Today's Topics:

   1. 2x3 slides (Pat Keller)
   2. Re: 2x3 slides (Pamela Marcum)
   3. lids for stainer containers (Perry, Margaret)
   4. RE: RE: Flame substitute for embedding (Bernice Frederick)
   5. Blue haze (Marshall, Kimberly)
   6. Re: PLP fixative lysine  (Gayle Callis)


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Message: 1
Date: Wed, 03 Dec 2008 11:29:55 -0600
From: Pat Keller <kellerp <@t> ent.wustl.edu>
Subject: [Histonet] 2x3 slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C55C1E33.50C1%kellerp <@t> ent.wustl.edu>
Content-Type: text/plain;	charset="US-ASCII"

Fisher has them


MILLIPORE CORP  MICROSCOPE SLIDES 2"X3" 72/PK

Fisher offers many products that do not appear in our catalogs. This may be one of those products, so pictures and detailed descriptions are not available. However, you may be able to order it by adding it to your shopping cart.
 

Item
Description     Catalog Number     Quantity     Price
MICROSCOPE SLIDES 2"X3" 72/PK
    XX1007615
Vendor No.:XX1007615         Pack of 72 for $51.26


Patricia Keller, 
Sr. Res. Tech.   Histology Core Manager
Washington University School of Medicine Department of Otolaryngology Campus box 8115
4566 Scott Ave.
St. Louis,   MO.   63110
KellerP <@t> ent.wustl.edu
http://www.otocore.org/

OFFICE    314-747-7166
FAX          314-747-7230


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Message: 2
Date: Wed, 3 Dec 2008 17:34:48 +0000 (UTC)
From: Pamela Marcum <mucram11 <@t> comcast.net>
Subject: Re: [Histonet] 2x3 slides
To: Pat Keller <kellerp <@t> ent.wustl.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<966250373.78611228325688567.JavaMail.root <@t> sz0001a.westchester.pa.mail.comcast.net>
	
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I got the slides from Thermo Fisher with some problems a long while ago.  The issue was finding a 100 slide box for storage and shipping.  I did order them this morning from Brain Research and they had stock of the boxes.  Thanks to everyone who helped and now I know where to go for the boxes next time. 



Pam Marcum 


----- Original Message -----
From: "Pat Keller" <kellerp <@t> ent.wustl.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 3, 2008 12:29:55 PM GMT -05:00 US/Canada Eastern
Subject: [Histonet] 2x3 slides 

Fisher has them 


MILLIPORE CORP  MICROSCOPE SLIDES 2"X3" 72/PK 

Fisher offers many products that do not appear in our catalogs. This may be one of those products, so pictures and detailed descriptions are not available. However, you may be able to order it by adding it to your shopping cart. 
  

Item
Description     Catalog Number     Quantity     Price MICROSCOPE SLIDES 2"X3" 72/PK     XX1007615 Vendor No.:XX1007615         Pack of 72 for $51.26 


Patricia Keller,
Sr. Res. Tech.   Histology Core Manager Washington University School of Medicine Department of Otolaryngology Campus box 8115
4566 Scott Ave. 
St. Louis,   MO.   63110
KellerP <@t> ent.wustl.edu
http://www.otocore.org/ 

OFFICE    314-747-7166
FAX          314-747-7230 


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Message: 3
Date: Wed, 3 Dec 2008 11:46:33 -0600
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] lids for stainer containers
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<FCA5EF47F9BC694CBB4C58FEA042196342F4F11F88 <@t> SDSU-MBX.jacks.local>
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We are looking for a source that sells a lid that looks like wax covered cardboard.  We use them on top of the Shandon slide containers holding xylene or formula 83 while waiting to coverslip.  They are about 5x7 and very thin.  They have been here for years so I have no idea where they came from.  Any help in finding them or a substitute will be greatly appreciated.  Thanks.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007



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Message: 4
Date: Wed, 3 Dec 2008 11:48:12 -0600
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] RE: Flame substitute for embedding
To: "'Jacqueline Farnsworth'" <Jacqueline.Farnsworth <@t> cls.ab.ca>,
	"'Celebre Julia'" <celebrej <@t> HHSC.CA>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c9556f$52b14590$d00f7ca5 <@t> lurie.northwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

We have a Shandon Paratrimmer, which melts the wax not only off blocks but
does a good job of keeping those forceps clean and hot.
We have a bacti-incinerator, but know we can get a tall flame if we leave
the paraffin coated forceps in there!!
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jacqueline
Farnsworth
Sent: Wednesday, December 03, 2008 9:44 AM
To: Celebre Julia; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Flame substitute for embedding

We also used to have an open flame while embedding!  Now, we simply have a
policy in place where after EACH cassette is embedded, the Techs MUST
thoroughly wipe their forceps with disposable Kleenex (and then dispose of
it!). Also, we use toothless forceps as much as possible to reduce the
possibility of cross contamination.


Jacqueline Farnsworth
Anatomic Pathology, Tech III
Foothills Medical Centre
Calgary Laboratory Services

Ph: 403-944-1578
Fax: 403-944-4748
P Please consider the environment before printing this email.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Celebre Julia
[celebrej <@t> HHSC.CA]
Sent: December 3, 2008 8:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Flame substitute for embedding

Hello Histoland!!

We have just been told  we are no longer allowed to use alcohol burners
while embedding and we are to find a safer substitute, something to do with
no liking an open flame in the lab.  Other than incinerators or 6 pairs of
forceps at each centre, we've run out of ideas so we are asking all you
experts out there what your current practice is.. oh.. this change needs to
be done by this Friday!!

Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
905-527-0271  ext 46179
email: celebrej <@t> hhsc.ca



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Message: 5
Date: Wed, 3 Dec 2008 12:47:34 -0500
From: "Marshall, Kimberly" <Kimberly.Marshall <@t> ahss.org>
Subject: [Histonet] Blue haze
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<C1A04A53C51BE149AA98408B31BC3045030E80BE <@t> LKMCXCH13.ADVENTISTCORP.NET>
Content-Type: text/plain; charset=iso-8859-1

Howdy all,
 
   I have just changed over to Surgi Path's H & E system.  With the latest fear of running out of hematoxylin and all my Pathologist wanted us to change over.  Its a great stain but we have a bad "haze"  in the background.  It is not hurting the tissue or DX at all, but to ME it looks awful.  Is there anyone else out there using this?   We are not using positive charged slides or anything added to my water bath.  Any suggestions????  Thanks in advance
 
Kimberly Marshall HT (ASCP)

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Message: 6
Date: Wed, 3 Dec 2008 10:56:21 -0700
From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Re: PLP fixative lysine 
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B1D2924BFA1B401FB687B3596A84042E <@t> DHXTS541>
Content-Type: text/plain;	charset="iso-8859-1"

For the PLP fixative, the full name for the lysine is L- lysine monohydrochloride.  Sigma cat# L5626. 

Gayle M. Callis
HTL(ASCP)HT,MT

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