[Histonet] IHC on fresh frozen

[FU,DONGTAO]

Hi, all

  Recently I did some IHC(chromagen methods) on mouse fresh frozen 
tissues, mainly using insulin antibody on pancreas. The image is 
much fuzzier compare to paraffin embedding tissue. And the 
staining also smeared to acinar cells which surround the islet.

  I airdried slide(>30min) and used a general acetone method(-20C 
5min) to fix the tissue before I did IHC.

  How can I get a relatively sharp staining on the fresh frozen 
tissue?Does anyone here have any experience on it? Any 
suggestions?

  Many thanks and have a nice day,

Ann