[Histonet] T Cell and B Cell Markers on Lymph Node Touch Preps

[Jan Shivers]

Works on canine and feline tissues:
CD3; LabVision; cat. # RB-9039; HIER with Dako Target Retrieval high pH 9.0 
(or 0.01M citrate buffer, pH 6.0, if used on liver tissue)
CD20; LabVision; cat. # RB-9013; no pretreatment necessary.

If you have formalin in your fixative, you'll need to do HIER on the CD3 
slides... though the high pH will most likely remove some of your cells.

If you use alcohol or acetone as your fixative, you won't need to do any 
HIER.

Jan Shivers

----- Original Message ----- 
From: "Woodward, Denise" <denise.woodward <@t> uconn.edu>
To: "Sandra Cheasty" <cheastys <@t> svm.vetmed.wisc.edu>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, December 01, 2008 3:12 PM
Subject: RE: [Histonet] T Cell and B Cell Markers on Lymph Node Touch Preps


In my experience, CD20 does not work in canine or feline tissues. Try
CD79a instead. I can't comment on the use of touch preps. With FFPE,
Dako TRS pH9 for 20 minutes works great.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sandra
Cheasty
Sent: Monday, December 01, 2008 10:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] T Cell and B Cell Markers on Lymph Node Touch Preps

Hello,

            Does anyone have experience with performing
Immunocytochemistry (CD3, CD20) on touch preps of canine and feline
lymph nodes, particularly the following:



Fixation: What reagent(s), what temperature, how long, immediately or
wait until air dried, storage post fixing



Antigen Retrieval: If formalin is part of the fixative, is there a
general rule for HIER on ICC specimens that relates to HIER on paraffin
sections of lymph nodes for CD3 and CD20?



Thank you so very much.







Sandra Cheasty

Histology & Necrcopsy Supervisor

UW-Madison

School of Veterinary Medicine















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