[Histonet] isopropyl

Alexandra Meinl alexandra.meinl <@t> gmail.com
Fri Aug 29 16:06:49 CDT 2008


Do you prefer isopropyl alcohol over ethanol?
I'm also thinking about using isopropyl for our specimens.
Isopropyl dehydrates more slowly, but how long is "more" when compared to
Are there any limitations (e.g. are there stainings that can't be done on
isopropyl dehydrated tissue)?



2008/8/29 Rene J Buesa <rjbuesa <@t> yahoo.com>

> It is an alcohol with three carbons, with a higher molecular weight (30%
> more) and with the alcohol group in the center carbon, slightly less
> miscible in water so less hydrophylic (dehydrates more slowly), but
> regardless of all this, if it works better for you, use it.
> René J.

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