[Histonet] isopropyl

[Rene J Buesa]

Perhaps the word "slowly" to characterize how isopropyl dehydrates is an "unfortunate" one, it would be "more gently" but the overall speed is the same.
Some instruments and technologies, like the used in the Peloris tissue processor, rest on the sole use of isopropyl. Dehydration has no noticeable impact in any staining.
René J.

--- On Fri, 8/29/08, Alexandra Meinl <alexandra.meinl <@t> gmail.com> wrote:

From: Alexandra Meinl <alexandra.meinl <@t> gmail.com>
Subject: Re: [Histonet] isopropyl
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 29, 2008, 5:06 PM

Hello,

Do you prefer isopropyl alcohol over ethanol?
I'm also thinking about using isopropyl for our specimens.
Isopropyl dehydrates more slowly, but how long is "more" when
compared to
ethanol?
Are there any limitations (e.g. are there stainings that can't be done on
isopropyl dehydrated tissue)?

Thanks,

Alex



2008/8/29 Rene J Buesa <rjbuesa <@t> yahoo.com>

> It is an alcohol with three carbons, with a higher molecular weight (30%
> more) and with the alcohol group in the center carbon, slightly less
> miscible in water so less hydrophylic (dehydrates more slowly), but
> regardless of all this, if it works better for you, use it.
> René J.
>
>
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