[Histonet] Antigen retrieval

[Victoria Baker]

Ahhhhh!  Now then I can relate to that!  I had a lab partner who left
a bunsen burner on one evening and the cleaning staff called me at
home to find out why it was on and then how to turn it off!!!  Fear
not - this is small stuff.  Best results wished for you.

Vikki

On 4/12/08, Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au> wrote:
> Hi Gayle, Renee and others,
>
> Thanks for the reply's. I am staining for KIM-1/TIM-1 and all the literature states that it has to be subjected to antigen retrieval at 80 degree's celsius overnight,- I was just worried that I would come back to the lab the next day to find either the lab had burned down or my tissue ruined!
>
> Kim
>
> Gayle Callis <gayle.callis <@t> bresnan.net> wrote:>
> > Rene and Kim,
> >
> > If you cover the waterbath (they come with covers) and also the slide
> > container (coplin jars, whatever), it should eliminate evaporation.
> >
> > Waterbath methods are also found in the literature.  Jules Elias has
> > published a method but I do not recall the temperature used overnight.
> >
> > I have several waterbath heated methods that use higher temperatures for
> > shorter times if Kim is interested. I will be happy to provide those via
> > private message with attachment.
> >
> > Gayle M. Callis
> > HTL/HT/MT(ASCP)
> > Bozeman MT 59715
> >
> >
> > ----- Original Message -----
> > From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
> > To: "Kim O'Sullivan" <Kim.Osullivan <@t> med.monash.edu.au>;
> > <histonet <@t> lists.utsouthwestern.edu>
> > Sent: Saturday, April 12, 2008 9:03 AM
> > Subject: Re: [Histonet] Antigen retrieval
> >
> >
> >> Never heard of such a procedure. On the other hand, unless you are
> >> dealing
> >> with a HUGE amount of buffer, it will evaporate during such a
> >> prolonged
> >> period of time.
> >>  Check your reference, I think something is not right.
> >>  René J.
> >>
> >> Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au> wrote:
> >>
> >>
> >>
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