[Histonet] Antigen retrieval

[Kim O'Sullivan]

Hi Gayle, Renee and others,

Thanks for the reply's. I am staining for KIM-1/TIM-1 and all the literature states that it has to be subjected to antigen retrieval at 80 degree's celsius overnight,- I was just worried that I would come back to the lab the next day to find either the lab had burned down or my tissue ruined!

Kim 

Gayle Callis <gayle.callis <@t> bresnan.net> wrote:> 
> Rene and Kim,
> 
> If you cover the waterbath (they come with covers) and also the slide
> container (coplin jars, whatever), it should eliminate evaporation.
> 
> Waterbath methods are also found in the literature.  Jules Elias has
> published a method but I do not recall the temperature used overnight.
> 
> I have several waterbath heated methods that use higher temperatures for
> shorter times if Kim is interested. I will be happy to provide those via
> private message with attachment.
> 
> Gayle M. Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
> 
> 
> ----- Original Message -----
> From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
> To: "Kim O'Sullivan" <Kim.Osullivan <@t> med.monash.edu.au>;
> <histonet <@t> lists.utsouthwestern.edu>
> Sent: Saturday, April 12, 2008 9:03 AM
> Subject: Re: [Histonet] Antigen retrieval
> 
> 
>> Never heard of such a procedure. On the other hand, unless you are
>> dealing
>> with a HUGE amount of buffer, it will evaporate during such a
>> prolonged
>> period of time.
>>  Check your reference, I think something is not right.
>>  René J.
>>
>> Kim O'Sullivan <Kim.Osullivan <@t> med.monash.edu.au> wrote:
>>
>>
>>
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