[Histonet]immuno stainers

[Nancy Lemke]

Hi All,
I have had a Nemesis for almost 2 years now and would be happy to answer any questions.  I run a histology core facility for a group of brain tumor researchers.  I stain  human only, animal only and human implanted into animal sections.  I use the Nemesis in the open mode only, no bar coding.  I use primarily Biocare reagents, but few Biocare antibodies.  I have done multiple stains on the instrument, including triple and quadruple.  I have no complaints about the instrument and find it versatile in every way, which is what I need.  All aspects of support are excellent, and I think that Biocare reagents, especially the newer Promark reagents are life savers to people staining xenografts, like myself.
I have had past extensive experience with the Biogenex i6000 and the Dako Immunostainer, so I have decent frames of reference.
I would be happy to respond to any questions.
Nancy

Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Sebree Linda A." LSebree <@t> uwhealth.org
Date: Thu, 13 Sep 2007 09:11:39 -0400
To: "Christine Tambasco" immrstambo <@t> hotmail.com
Subject: RE: [Histonet]immuno stainers

> Please post replies to Histonet.
> 
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-3224
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of > Christine Tambasco
> Sent: Wednesday, September 12, 2007 4:26 PM
> To: histomike <@t> charter.net; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet]immuno stainers
> 
> 
> 
>    Anybody  have  an  experiences  with  the  Nemisis from BioCare? > Would
>    appreciate any feed back. Thanks
> 
>    Christine Tambasco, HT (ASCP)
> 
>    St Marys Hospital, Amsterdam NY
>        ______________________________________________________________
> 
>      From:  "Mike Schlicht" <histomike <@t> charter.net>
>      To:  <histonet <@t> lists.utsouthwestern.edu>
>      Subject:  Re: [Histonet] Special Stainers
>      Date:  Wed, 12 Sep 2007 12:51:34 -0700
>      >We  are  currently  using  the DAKO Artisan for silver stains, and
>      have
>      >some  problems  with  uneven  staining every now and then, usually
>      when
>      >the dispensers are close to being empty, so we stop using the kit
>      >when there are 5 tests remaining to help reduce some of the
>      >problems.  We had a demo of the Nexus stainer, and found that the
>      >result were about the same.
>      >Mike
>      >San Luis Obispo, CA
>      >
>      >
>      >>Message: 15
>      >>Date: Tue, 11 Sep 2007 18:32:37 -0500
>      >>From: "Rebecca Johnson" <raj <@t> bluemarble.net>
>      >>Subject: [Histonet] Special Stainers
>      >>To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
>      >>Message-ID: <011a01c7f4cc$09fefcc0$7b48f9d8 <@t> CHURCH>
>      >>Content-Type: text/plain; charset="iso-8859-1"
>      >>
>      >>What special stainers is everyone using?  I now have a Ventana
>      >>Nexus. Bought in 1999.  I know this is old, but it has been
>      >>maintained well. We have had problems with it several times over
>      >>the years. Mostly with sliver stains or uneven staining and no
>      >>staining.  I would like to know what others are using and your
>      >>experiences are. Thanks for all you help.
>      >>raj
>      >>
>      >
>      >
>      >_______________________________________________
>      >Histonet mailing list
>      >Histonet <@t> lists.utsouthwestern.edu
>      >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>      _________________________________________________________________
> 
>    [1]Kick  back  and  relax  with  hot  games and cool activities at > the
>    Messenger Café.
> 
> References
> 
>    1. http://g.msn.com/8HMBENUS/2740??PS=47575
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


==============================================================================
CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies.
 
Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us.

==============================================================================