[Histonet] Need help with Brain Tumor FS

[Joe Nocito]

have you tried liquid nitrogen?

JTT
----- Original Message ----- 
From: <tdobersztyn <@t> chmca.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 13, 2007 3:14 PM
Subject: [Histonet] Need help with Brain Tumor FS


> Hello All!
> 
> Can anyone recommend a protocol for frozen section technique - to cut 
> Brain tumor biopsies.
> 
> The usual ice-crystal artifact sometimes renders the Frozen Sections 
> unreadable for intra-operative diagnosis.
> 
> We use a Leica UV1850 cryostat set at -21*
> OCT media 
> and cut our FS at 8 microns
> then:
> rapid stain H/E
> 
> The pathologist here tends to believe it is our technique that produces 
> the artifact.
> 
> fast freezing with spray  vs slow freezing??????????
> 
> We have tried a lot of methods to eliminate this problem 
> 
> HELP!!!!!!!!!!! I know someone out there has a remedy for the headache 
> this has created!!!!
> 
> 
> Thank you all in advance!!! 
> I am glad I can pose questions to others who might be dealing with the 
> issues I deal with too!
> 
> Theresa
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