[Histonet] Re: Need help with Brain Tumor FS

[Gayle Callis]

Theresa,

Joe's suggestion on snap freezing is excellent to avoid freezing artifact, 
but you need to discuss this with Nancy Lemke at 
Nperson211 <@t> comcast.net.   She also works with brain tumor biopsies and has 
experienced your problems too.  She can give you even more hints since she 
is involved with brain tissue biopsies for both clinical and research. She 
can also provide information on optimal temperature setting for these 
biopsies/tumor samples.

If Nancy has already replied, please forgive me, I have been off Histonet 
for weeks.

Good luck

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610



At 03:26 PM 9/13/2007, you wrote:
>have you tried liquid nitrogen?
>
>JTT
>----- Original Message ----- From: <tdobersztyn <@t> chmca.org>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Thursday, September 13, 2007 3:14 PM
>Subject: [Histonet] Need help with Brain Tumor FS
>
>
>>Hello All!
>>Can anyone recommend a protocol for frozen section technique - to cut 
>>Brain tumor biopsies.
>>The usual ice-crystal artifact sometimes renders the Frozen Sections 
>>unreadable for intra-operative diagnosis.
>>We use a Leica UV1850 cryostat set at -21*
>>OCT media and cut our FS at 8 microns
>>then:
>>rapid stain H/E
>>The pathologist here tends to believe it is our technique that produces 
>>the artifact.
>>fast freezing with spray  vs slow freezing??????????
>>We have tried a lot of methods to eliminate this problem
>>HELP!!!!!!!!!!! I know someone out there has a remedy for the headache 
>>this has created!!!!
>>