[Histonet] mouse diaphrams

[Molinari, Betsy]

It is not Friday but I can see the remarks a mile away on this one.
 I may have posted this before and I apologize if I have.
I am trying to freeze some mouse diaphragms. I continue to get artifact
(holes).I have tried freezing on dry ice, dry ice/isopentane slurry,
isopentane, Isopentane/ methlybutane . I have blotted the tissue to
remove excess moisture all to no avail. Any suggestions?
Thanks in advance...should be wild time in Denver w/ World Series going
on. I understand that on that Monday the W.S. and Monday night football
will be happening at the same time right next door to each other,
Betsy
 
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812