[Histonet] anti-macrophage marker reactive to mouse
Melissa Mazan
melissa.mazan <@t> tufts.edu
Mon Oct 15 13:22:29 CDT 2007
Hi all,
I'm looking for an anti-mouse anti-macrophage marker that is NOT raised
in mouse for use in immunohistochemistry (FFPE sections). Most of the
antibodies that I've used in the past - CD45, Thy-1, CD11 - are too
nonspecific being for anti-hematopoietic cells. Can anyone give a
suggestion? Thanks - Melissa
histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Jobs in Ireland (mariakatleba <@t> aol.com)
> 2. Collecting PBMCs (annamaria touvra)
> 3. Histo Tech and Cyto Tech needed in the San Francisco Bay
> Area. Can you help? (Pam Barker)
> 4. RE: CRYOSTAT TROUBLESHOOTING (Alan Bright)
> 5. IEC manual and specimen holder (Neil Schmitzer-Torbert)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 14 Oct 2007 21:19:37 -0400
> From: mariakatleba <@t> aol.com
> Subject: [Histonet] Jobs in Ireland
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8C9DCE09612B69B-E8C-92D <@t> WEBMAIL-MB10.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Anyone know how I can look for histology jobs in Ireland???
>
> Thanks in advance
>
> Maria Katleba MS HT(ASCP)
> Napa California USA
> ________________________________________________________________________
> Email and AIM finally together. You've gotta check out free AOL Mail! - http://mail.aol.com
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 15 Oct 2007 02:30:26 -0700 (PDT)
> From: annamaria touvra <atouvr <@t> yahoo.com>
> Subject: [Histonet] Collecting PBMCs
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <840665.38722.qm <@t> web60712.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
> Goodmorning everyone,
>
> I would like some help concerning the collection/separation of Peripheral Mononuclear Cells (PBMCs) from whole blood.
> If anyone has done it with HIstopaque-1077 of Sigma-Aldrich, I would like to know which is the appropriate ratio of Histopaque and whole blood, at what temperature is the centrifugation done, how many rpm are used and for how long. The procedure mentions to use 15ml centrifuge tubes. What will happen if I'll use tubes of 5 or 10 ml?
> If there is anything else making the separation better I would appreciate any comment.
>
> Thank you in advance,
>
> Have a nice week full of joy in work,
> Anna-Maria
>
>
>
> ---------------------------------
> Pinpoint customers who are looking for what you sell.
>
> ------------------------------
>
> Message: 3
> Date: Mon, 15 Oct 2007 09:41:02 -0400
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] Histo Tech and Cyto Tech needed in the San
> Francisco Bay Area. Can you help?
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1IhQBp-0004Ff-4q <@t> elasmtp-scoter.atl.sa.earthlink.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Histonetters,
> I hope you all had great weekends. I am presently on a search for one
> of my best clients that is in need of a Cyto Tech and a Histo Tech in
> the San Francisco Bay area. My client offers excellent compensation and
> relocation and offers an environment that is great to work in.
>
>
>
> The help I need is do you know anyone that might be interested in
> hearing about either of these opportunites? If so could you please
> forward my e-mail to them?
>
>
>
> If you are interested in the histo tech position or know a cyto tech
> that might be interested in the cytotechnologist position please call me
> ASAP at 866-607-3542 or e-mail me at relia1 <@t> earthlink.net
>
>
>
> Thank you,
>
>
> Thank You!
>
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: relia1 <@t> earthlink.net
> <http://home.earthlink.net/~relia1>
> www.myspace.com/pamatrelia
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 15 Oct 2007 15:37:13 +0100
> From: "Alan Bright" <abright <@t> brightinstruments.com>
> Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING
> To: "Stephen Peters M.D." <petepath <@t> yahoo.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B7C44C9CD907BF4580B74C4B02C5582EEC217F <@t> bright-sbs.Bright.local>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Dear Dr. Peters,
>
> There is no need to be saddened by the lack of interest of cryostat
> manufactures as we have a constant devotion to cryostat developments
> spanning over 50 years.
> We developed and made available for our cryostats over 14 years ago, 10
> years ahead of you, a very superior system for face down tissue
> embedding, using frozen object holders and much faster freezing at -50
> degs. C.
>
> I for one do not hope you fade away as we continually consult with users
> to establish new techniques and products to advance frozen sectioning.
> Perhaps when looking into future developments you would consider
> contacting us.
>
> Best Regards
>
> Alan Bright
>
> Bright Instrument Co.Ltd.
> St Margaret's Way
> Huntingdon
> Cambridgeshire
> PE29 6EU
> England
>
> Tel No:+44 (0)1480 454528
> Fax No:+44 (0)1480 456031
> Email: abright <@t> brightinstruments.com
> Web Site: www.brightinstruments.com
> Skype: dazzle0
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephen
> Peters M.D.
> Sent: 11 October 2007 19:32
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] CRYOSTAT TROUBLESHOOTING
>
> For the last 5 years I have been promoting a system of face down
> embedding tissue
> for frozen section which I developed over my 25 year surgical
> pathology practice.
> The system is rapid, very precise and wastes very little tissue. I am
> all but exhausted devoting most of my vacation and free time to bring
> this sytem to my colleagues at very limited profit.My chucks are made
> y.to be used cold with deep wide
> waffle pattern grooves. They are made of stainless so they function
> as a heat extractor.
> With the exception of Leica, I am quite surprised and saddened by the
> lack of interest in
> our cryostat manufacturers to explore a new technology which could
> benefit their customers. I believe they are hoping I fade away. If you
> want to improve your frozen section practice, I suggest you seek out
> this system. I would be happy to direct you to it, but the last time I
> offered help, one of the vendors complained I was breaking a vendor
> rule.
>
> Stephen
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>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 15 Oct 2007 11:57:16 -0400
> From: "Neil Schmitzer-Torbert" <torbertn <@t> wabash.edu>
> Subject: [Histonet] IEC manual and specimen holder
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003501c80f44$0ecdcbe0$722b20a1 <@t> torbertnPC>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello,
>
>
>
> I have recently come into possession of a IEC CTD cryostat with a minot
> microtome. I am hoping to find a copy of the manual for the cryostat and
> somewhere that I can purchase a specimen holder for the microtome. Any
> advice on where I can find these items would be greatly appreciated!
>
>
>
> Neil Schmitzer-Torbert
>
>
>
> BKT Assistant Professor of Psychology
>
> Wabash College
>
> Crawfordsville, IN 47933
>
> (765) 361-6076
>
>
>
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 47, Issue 17
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