[Histonet] anti-macrophage marker reactive to mouse

[Liz Chlipala]

You can use either F4/80 or CD68 from serotec.  Both work in FFPE
tissues.  They are rat anti-mouse antibodies. I have protocols if you
need them.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Melissa
Mazan
Sent: Monday, October 15, 2007 12:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] anti-macrophage marker reactive to mouse

Hi all,
I'm looking for an anti-mouse anti-macrophage marker that is NOT raised
in mouse for use in immunohistochemistry (FFPE sections).  Most of the
antibodies that I've used in the past - CD45, Thy-1, CD11 - are too
nonspecific being for anti-hematopoietic cells.  Can anyone give a
suggestion? Thanks - Melissa

histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
>    1. Jobs in Ireland (mariakatleba <@t> aol.com)
>    2. Collecting PBMCs (annamaria touvra)
>    3. Histo Tech and Cyto Tech needed in the San Francisco Bay
>       Area. Can you help? (Pam Barker)
>    4. RE: CRYOSTAT TROUBLESHOOTING  (Alan Bright)
>    5. IEC manual and specimen holder (Neil Schmitzer-Torbert)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 14 Oct 2007 21:19:37 -0400
> From: mariakatleba <@t> aol.com
> Subject: [Histonet] Jobs in Ireland
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8C9DCE09612B69B-E8C-92D <@t> WEBMAIL-MB10.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Anyone know how I can look for histology jobs in Ireland???
>
> Thanks in advance
>
> Maria Katleba MS HT(ASCP)
> Napa California USA
> ______________________________________________________________________
> __ Email and AIM finally together. You've gotta check out free AOL 
> Mail! - http://mail.aol.com
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 15 Oct 2007 02:30:26 -0700 (PDT)
> From: annamaria touvra <atouvr <@t> yahoo.com>
> Subject: [Histonet] Collecting PBMCs
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <840665.38722.qm <@t> web60712.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
>
> Goodmorning everyone,
>    
>   I would like some help concerning the collection/separation of
Peripheral Mononuclear Cells (PBMCs) from whole blood.
>   If anyone has done it with HIstopaque-1077 of Sigma-Aldrich, I would
like to know which is the appropriate ratio of Histopaque and whole
blood, at what temperature is the centrifugation done, how many rpm are
used and for how long. The procedure mentions to use 15ml centrifuge
tubes. What will happen if I'll use tubes of 5 or 10 ml?
>   If there is anything else making the separation better I would
appreciate any comment.
>    
>   Thank you in advance,
>    
>   Have a nice week full of joy in work,
>   Anna-Maria
>
>
>        
> ---------------------------------
> Pinpoint customers who are looking for what you sell. 
>
> ------------------------------
>
> Message: 3
> Date: Mon, 15 Oct 2007 09:41:02 -0400
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] Histo Tech and Cyto Tech needed in the San
> 	Francisco Bay	Area. Can you help?
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1IhQBp-0004Ff-4q <@t> elasmtp-scoter.atl.sa.earthlink.net>
> Content-Type: text/plain;	charset="iso-8859-1"
>
> Hi Histonetters,
> I hope you all had great weekends.  I am presently on a search for one

> of my best clients that is in need of a Cyto Tech and a Histo Tech  in

> the San Francisco Bay area.  My client offers excellent compensation 
> and relocation and offers an environment that is great to work in.
>
>  
>
> The help I need is do you know anyone that might be interested in 
> hearing about either of  these opportunites?  If so could you please 
> forward my e-mail to them?
>
>  
>
>  If you are interested in the histo tech  position or know a cyto tech

> that might be interested in the cytotechnologist position please call 
> me ASAP at 866-607-3542 or e-mail me at relia1 <@t> earthlink.net
>
>  
>
> Thank you,
>
>
> Thank You!
>  
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell:     (407)353-5070
> FAX:     (407)678-2788
> E-mail: relia1 <@t> earthlink.net
> <http://home.earthlink.net/~relia1>
> www.myspace.com/pamatrelia
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 15 Oct 2007 15:37:13 +0100
> From: "Alan Bright" <abright <@t> brightinstruments.com>
> Subject: RE: [Histonet] CRYOSTAT TROUBLESHOOTING
> To: "Stephen Peters M.D." <petepath <@t> yahoo.com>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 	<B7C44C9CD907BF4580B74C4B02C5582EEC217F <@t> bright-sbs.Bright.local>
> Content-Type: text/plain;	charset="us-ascii"
>
>
> Dear Dr. Peters,
>
> There is no need to be saddened by the lack of interest of cryostat 
> manufactures as we have a constant devotion to cryostat developments 
> spanning over 50 years.
> We developed and made available for our cryostats over 14 years ago, 
> 10 years ahead of you, a very superior system for face down tissue 
> embedding, using frozen object holders and much faster freezing at -50

> degs. C.
>
> I for one do not hope you fade away as we continually consult with 
> users to establish new techniques and products to advance frozen
sectioning.
> Perhaps when looking into future developments you would consider 
> contacting us.
>
> Best Regards
>
> Alan Bright
>
> Bright Instrument Co.Ltd.
> St Margaret's Way
> Huntingdon
> Cambridgeshire
> PE29 6EU
> England
>
> Tel No:+44 (0)1480 454528
> Fax No:+44 (0)1480 456031
> Email: abright <@t> brightinstruments.com
> Web Site: www.brightinstruments.com
> Skype: dazzle0
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Stephen Peters M.D.
> Sent: 11 October 2007 19:32
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] CRYOSTAT TROUBLESHOOTING
>
> For the last 5 years I have been promoting a system of face down 
> embedding tissue
>    for frozen section which I developed over my 25 year surgical 
> pathology practice.
>   The system is rapid, very precise and wastes very little tissue. I 
> am all but exhausted devoting most of my vacation and free time to 
> bring this sytem to my colleagues at very limited profit.My chucks are

> made y.to be used cold with deep wide
>    waffle pattern grooves. They are made of stainless so they function

> as a heat extractor.
>   With the exception of Leica, I am quite surprised and saddened by 
> the lack of interest in
>    our cryostat manufacturers to explore a new technology which could 
> benefit their customers. I believe they are hoping I fade away. If you

> want to improve your frozen section practice, I suggest you seek out 
> this system. I would be happy to direct you to it, but the last time I

> offered help, one of the vendors complained I was breaking a vendor 
> rule.
>    
>   Stephen
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 15 Oct 2007 11:57:16 -0400
> From: "Neil Schmitzer-Torbert" <torbertn <@t> wabash.edu>
> Subject: [Histonet] IEC manual and specimen holder
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003501c80f44$0ecdcbe0$722b20a1 <@t> torbertnPC>
> Content-Type: text/plain;	charset="US-ASCII"
>
> Hello,
>
>  
>
> I have recently come into possession of a IEC CTD cryostat with a 
> minot microtome.  I am hoping to find a copy of the manual for the 
> cryostat and somewhere that I can purchase a specimen holder for the 
> microtome.  Any advice on where I can find these items would be
greatly appreciated!
>
>  
>
> Neil Schmitzer-Torbert
>
>  
>
> BKT Assistant Professor of Psychology
>
> Wabash College
>
> Crawfordsville, IN  47933
>
> (765) 361-6076
>
>  
>
>
>
> ------------------------------
>
> _______________________________________________
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> End of Histonet Digest, Vol 47, Issue 17
> ****************************************
>   

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