AW: [Histonet] H&E Staining

[Gudrun Lang]

Make the washing steps very short and begin with 96% alcohol, if you use
alcoholic eosin. (15-30 sek. 96%-100%-100%).

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von William
Ares
Gesendet: Mittwoch, 10. Oktober 2007 15:25
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] H&E Staining

 i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded
tissues and keep having the eosin wash out during the dehydration steps
(stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this
would happen?  am i missing a step in between that allows the stain to
'stick'?  thanks


William Ares
Research Technician
Laboratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University
Tel: (212) 746 5056
Email: wia2005 <@t> med.cornell.edu


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