[Histonet] H&E Staining

[William Ares]

 i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded tissues and keep having the eosin wash out during the dehydration steps (stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this would happen?  am i missing a step in between that allows the stain to 'stick'?  thanks


William Ares
Research Technician
Laboratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University
Tel: (212) 746 5056
Email: wia2005 <@t> med.cornell.edu