[Histonet] Giemsa Stain

[Marshall Terry Dr, Consultant Histopathologist]

So we are singing from the same hymn sheet....
Stains can't have internal controls.
Tissues can, in relation to some stains.

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
koellingr <@t> comcast.net
Sent: 14 November 2007 13:43
To: Rene J Buesa; Schaundra Walton; Histonet
Subject: Re: [Histonet] Giemsa Stain

Rene,
Are you saying a histochemical stain for an organism (Helicobactor, AFB,
spirochetes, etc) must have a positive control with which I'd agree?
But are you saying that for "any" HC stain like elastic, trichrome, PAS,
alcian blue, etc that internal controls don't (cannot) even exist?
Thanks,

Ray Koelling
PhenoPath Labs
Seattle, WA

-------------- Original message --------------
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

> Giemsa, as any other stain, is just that, a stain, and stains do not 
> (cannot) have "internal controls".
> As with any other HC procedure you have to use a positive control 
> along with your tissue.
> To completely demonstrate that your Giemsa procedure worked as 
> expected, you coul use a blood smear or an appendix section as 
> controls. That will depend on your pathologist's whims.
> Ren$B!&(BJ. 
> 
> Schaundra Walton wrote: 
> We've been using a Giemsa stain for our H. pylori cases without a 
> control tissue. It has come to my attention that this may not be good 
> practice. I was under the impression that the Giemsa stain had an 
> internal control. Is anyone else using a this stain for H. pylori? Are
you running a tissue control with it?
> 
> 
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