[Histonet] Artefacts

[John Kiernan]

Dear Toxicology <dis.tox <@t> suven.com>
 
You need to explain your problem more clearly. Please include histonet <@t> lists.utsouthwestern.edu
in your replies. We all want to share our knowledge.
 
What tissue are you staining? How was it fixed?  Are you staining dewaxed and hydrated paraffin sections?  Cryostat sections?  
 
Which haemalum & eosin method did you use?  Does your lab have a book with instructions for H&E staining?  If not, why not?
 
Are the "faint stained spots on sections" blue, purple, pink or red? Where are these spots?  
 
John Kiernan
Anatomy, UWO
London, Canada.
===
----- Original Message -----
From: Toxicology <dis.tox <@t> suven.com>
Date: Thursday, November 8, 2007 1:36
Subject: [Histonet] Artefacts
To: histonet <@t> lists.utsouthwestern.edu

> Dear all,
> I am facing a problem in Hematoxylin and Eosin staining. There 
> are several 
> faint stained spots on sections and because of that its 
> difficuly to 
> interpret the lesions.  I am unable to sort out the 
> problem. Can any one 
> help me?
>  
> 
> 
> 
> -------------------------Email Disclaimer------------------------
> ---------
>