[Histonet] Clone for Prog. Receptor

Sat Nov 10 10:10:13 CST 2007

In my experience, the rab monoclonals for er and pr are just as sensitive or
more so than the ms monoclonal, both using the same labeled polymer
detection.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Thursday, November 08, 2007 11:38 AM
To: 'Drew Sally A.'; 'Histonet'
Subject: RE: [Histonet] Clone for Prog. Receptor

Per a lecture at the NSh mouse monocloncal antibodies have better affinity
than rabbit monoclonals. We use 1A6 but then, we are following a clinical
protocol and cannot deviate.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Drew Sally
A.
Sent: Thursday, November 08, 2007 11:07 AM
To: Histonet
Subject: [Histonet] Clone for Prog. Receptor

We currently are using the 1A6 clone for our progesterone receptor(PR)
IHC.
We were wondering how other people felt about rabbit monoclonal PR
antibodies.
Does anyone have strong preferences or experiences with other PR
clones-mouse or rabbit-that 
they'd like to share? 
   Thank you...!

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
(608)265-6596

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