[Histonet] Artefacts

Marshall Terry Dr, Consultant Histopathologist Terry.Marshall <@t> rothgen.nhs.uk
Thu Nov 8 11:13:21 CST 2007

Some of these questions should be answered by the photos, but I cannot
find them.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John
Sent: 08 November 2007 17:11
To: Toxicology
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Artefacts

Dear Toxicology <dis.tox <@t> suven.com>
You need to explain your problem more clearly. Please include
histonet <@t> lists.utsouthwestern.edu in your replies. We all want to share
our knowledge.
What tissue are you staining? How was it fixed?  Are you staining
dewaxed and hydrated paraffin sections?  Cryostat sections?  
Which haemalum & eosin method did you use?  Does your lab have a book
with instructions for H&E staining?  If not, why not?
Are the "faint stained spots on sections" blue, purple, pink or red?
Where are these spots?  
John Kiernan
Anatomy, UWO
London, Canada.
----- Original Message -----
From: Toxicology <dis.tox <@t> suven.com>
Date: Thursday, November 8, 2007 1:36
Subject: [Histonet] Artefacts
To: histonet <@t> lists.utsouthwestern.edu

> Dear all,
> I am facing a problem in Hematoxylin and Eosin staining. There are 
> several faint stained spots on sections and because of that its 
> difficuly to interpret the lesions.  I am unable to sort out the 
> problem. Can any one help me?
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